Upon the successful completion of this course, the participants will have an understanding of Principles and practices of the modern instrument’s technique

The course is of interest for any person working in any laboratory. The course for chemists, laboratory technicians, chemical engineers, instrument engineers and supervisors who work in laboratory

This interactive Training will be highly interactive, with opportunities to advance your opinions and ideas and will include;

• Lectures
• Workshop & Work Presentation
• Case Studies and Practical Exercise
• Videos and General Discussions

Day 1:   Spectroscopic Instrumental Analysis

• Introduction
• Analytical Chemistry Historical and review
• Basic Principle and theory of Analytical Chemistry
• Analytical Chemistry and Chemical Analysis
• Type of Chemical Analysis Methods
  • Classical Methods
  • Instrumental Methods

Spectroscopic Instrumental Analysis

• Historical and review
• Basic Principals
• Electromagnetic Spectrum
• Beer–Lambert Law
• Theory of Spectroscopy
  • Absorption
  • Emission
  • Fluorescence
  • Phosphorescence
• Type of Spectroscopy Analysis
  • Molecular Spectroscopy analysis
  • Atomic Spectroscopy analysis

Day 2: UV-Vis Molecular Spectroscopy & X-RAY

• Instrumentation
• Monochromator
• Photomultiplier tube (PMT) detector
• Single beam
• Double beam
• Operation and maintenance

• Application for trace metals with UV-Vis Molecular Spectroscopy
  • Water samples
  • Organic samples
  • Solid samples
• X-RAY Instrumental Analysis
  • X-ray florescence XRF
  • X-ray Diffraction XRD
  • Application for solid samples
  • Application for liquid samples

Day 3:   Atomic Absorption Spectroscopy (AAS)

• AAS Instrumentation & components
• Effect of Temperature on Absorption
• AAS Instrumentation:
  • Atomization
  • Flame AAS
  • Graphite Furnace AAS
  • Radiation Sources
  • Hollow-Cathode Lamps (HCL)
  • Electrodeless Discharge Lamps (EDL)
  • Optical System
  • Single beam
  • Double beam
  • Mono-chrometor
  • Detector
  • Photomultiplier tube (PMT)
  • Charged Coupled Device (CCD)
  • Computer interface
• AAS; Advantages and Disadvantages
• AAS; Maintenance & Troubleshooting
  • Routine Maintenance
  • Problem and troubleshot
  • Safety requirement for running
  • Interferences (Spectral, Chemical Ionization (
  • Background Correction
  • Deuterium lamp correction
  • Zeeman correction

 Day 4:   Inductively Coupled Plasma (ICP)

• Introduction
• Theory
• Different type of Plasma Techniques
• Instrumentation
  • Sample introduction system (Nebulizer)
  • Peristaltic pump
  • ICP torch (Radial View & Axial View)
  • Radio frequency (RF) generator
  • Load Coils
  • Torches
  • Transfer optics and spectrometer
  • Monochromator- and Polychromator
  • Detectors (PMT, CID, CCD, CMOS)
  • Computer interface
• Routine Maintenance and troubleshot
• ICP Advantages and Disadvantages
• Safety requirement for running ICP
• Comparing ICP with Atomic Spectroscopic Techniques
• Sampling, Preparation and Treatment
• Analysis Techniques
• ICP & AAS with LIMS
• Applications of Spectroscopy (ICP &AAS)
  • Water & wastewater analysis
  • Environmental analysis
  • Oil and Hydrocarbon analysis
  • Metallurgical analysis

Day 5:   Quality Control & Quality Assurance

• Laboratory Data Results
• Recorders & Data Acquisition and analysis
• Laboratory Report
• Analytical Method validation
• Detection Limits (LOD, LOQ, LOL,…)
• Types of calibration methods
  • Standard calibration
  • Standard addition calibration
  • Internal standard calibration
• Evaluation of analytical data
• Study Performance and Reporting
• Errors in Qualitative and Quantitative Analysis
• Correction of errors and improving accuracy
• Reputability, Reducibility
• Quality Assurance Program
• Measurement Uncertainty in chemical measurements
• Quality Audit (Internal and External)

Practical application and case study for trace metals analysis:

• Sample Handling and prepare sample for analysis
• Prepare standard solution of metals for different concentration
• Make standard calibration for AAS, and ICP with standard solutions
• Make analysis of metals by ICP and AAS reference to standard methods
• Make analysis of metals by X-RAY

RECTUS attendance certificate will be issued to all attendees completing minimum of 80% of the total course duration