The quality of analysis depends more on the quality of the operator than that of the instrument, so this course will help laboratory staff   to understand meaning of spectroscopy, and to be apple to make elemental analysis with high sensitivity by spectroscopy, upon the successful completion of this course, the participants will have an understanding of Principles and practices of the modern instrument’s technique

The course creates for chemists, laboratory technicians, instrument engineers and supervisors who work in laboratory.

Day 1

• Introduction
• Analytical Chemistry Historical and review
• Basic Principle and theory of Analytical Chemistry
• Analytical Chemistry and Chemical Analysis
• Type of Chemical Analysis Methods
  • Classical Methods
  • Instrumental Methods
• Electromagnetic Spectrum
• Beer–Lambert Law
• Theory of Spectroscopy
  • Absorption
  • Emission
  • Fluorescence
  • Phosphorescence
• Type of Spectroscopy Analysis
  • Molecular Spectroscopy analysis
  • Atomic Spectroscopy analysis

Day 2

UV-Vis Molecular Spectroscopy

• • Instrumentation
  • Monochromator
  • Photomultiplier tube (PMT) detector
  • Single beam
  • Double beam
  • Operation and maintenance
• Application for trace metals with UV-Vis Molecular Spectroscopy
• • Water samples
  • Organic samples
  • Solid samples

Day 3

Atomic Absorption Spectroscopy (AAS)

• AAS Instrumentation:
  • Atomization
    • Flame AAS
    • Graphite Furnace AAS
  • Radiation Sources
    • Hollow-Cathode Lamps (HCL)
    • Electrodeless Discharge Lamps (EDL)
  • Optical System
    • Single beam
    • Double beam
    • Monochromator
  • Detector
    • Photomultiplier tube (PMT)
    • Charged Coupled Device (CCD)
  • Computer interface
• AAS; Advantages and Disadvantages
• AAS; Maintenance & Troubleshooting
  • Routine Maintenance
  • Problem and troubleshot
  • Safety requirement for running
  • Interferences (Spectral, Chemical Ionization )
  • Background Correction
    • Deuterium lamp correction
    • Zeeman correction

Day 4

Inductively Coupled Plasma (ICP)

•  Instrumentation
  • Sample introduction system (Nebulizer)
  • Peristaltic pump
  • ICP torch (Radial View & Axial View)
  • Radio frequency (RF) generator
  • Load Coils
  • Torches
  • Transfer optics and spectrometer
  • Monochromator- and Polychromator
  • Detectors (PMT, CID, CCD, CMOS)
  • Computer interface
• ICP Advantages and Disadvantages
• Comparing ICP with Atomic Spectroscopic Techniques
• Sampling, Preparation and Treatment
• Analysis Techniques
• ICP & AAS with LIMS
• Applications of Spectroscopy (ICP &AAS)

Day 5

Practical application and case study for trace metals analysis:

• Sample Handling and prepare sample for analysis
• Prepare standard solution of metals for different concentration
• Make standard calibration for AAS, and ICP with standard solutions
• Make analysis of metals by ICP and AAS reference to standard methods

Quality Control and Quality Assurance

• Laboratory Data Results
• Recorders & Data Acquisition and analysis
• Laboratory Report
• Analytical Method validation
• Detection Limits (LOD, LOQ, LOL,….)
• Types of calibration methods
  • Standard calibration
  • Standard addition calibration
  • Internal standard calibration
• Evaluation of analytical data
• Study Performance and Reporting
• Errors in Qualitative and Quantitative Analysis
• Correction of errors and improving accuracy
• Reputability, Reducibility
• Quality Assurance Program
• Measurement Uncertainty in chemical measurements
• Quality Audit (Internal and External)

RECTUS certificate will be issued to all attendees completing minimum of 75% of the total tuition hours of the WORKSHOP.